ABSTRACT
Using of high throughput sequencing technology to study the microbial diversity in complex samples has become one of the hottest issues in the field of microbial diversity research. In this study, the soil and sheep rumen chyme samples were used to extract DNA, respectively. Then the 25 ng total DNA was used to amplify the 16S rRNA V3 region with 20, 25, 30 PCR cycles, and the final sequencing library was constructed by mixing equal amounts of purified PCR products. Finally, the operational taxonomic unit (OUT) amount, rarefaction curve, microbial number and species were compared through data analysis. It was found that at the same amount of DNA template, the proportion of the community composition was not the best with more numbers of PCR cycle, although the species number was much more. In all, when the PCR cycle number is 25, the number of species and proportion of the community composition were the most optimal both in soil or chyme samples.
Subject(s)
Animals , Bacteria , Classification , DNA, Bacterial , Genetics , Gene Library , High-Throughput Nucleotide Sequencing , Polymerase Chain Reaction , Methods , RNA, Ribosomal, 16S , Genetics , Rumen , Microbiology , Sheep , Soil MicrobiologyABSTRACT
Objective To evaluate clinical effects of vacuum sealing drainage ( VSD ) and conventional dressing in treatment of diabetic foot(DF)ulcers.Methods We searched PubMed, CNKI and WanFang data, and collected the Chinese and English randomized controlled studies on VSD and conventional dressing in treat-ment of DF ulcers.The retrieval time was from Jan.2000 to Jan.2014.RevMan5.2 software was used to make Meta-analysis.Results Seven papers including 646 cases met the standard and Meta analysis was conducted ac-cording to these data.The results showed that compared with conventional dressing, VSD treatment shortened the healing time, increased the complete healing rate, reduced hospital stay, and reduced hospital costs and seconda-ry amputation rates.The difference had statistical significance(P<0.05).Conclusion VSD is superior to con-ventional dressing method in terms of the healing time, healing rate, hospital stay, hospital costs and amputation rate.